WT1和FGF9基因在克隆猪隐睾中mRNA表达和DNA甲基化状态分析

doi:10.11843/j.issn.0366-6964.2014.06.009

Abstract

Abstract:

In order to find out the possible causes of the cryptorchidism in cloned pigs which produced by our research group.The RT-PCR techniques and bisulfite salt sequencing analysis(BSP) was used to analysis the expression and promoter methylation status of the promoter CpG sites of WT1 and FGF9 genes in the testis of cloned boars.The results showed that，the expression of both genes in cloned pig C1 and C2 were higher than those of control group N，expression of WT1 and FGF9 genes in cloned pig C1 was much higher (3.49，9.83 vs 1.00).Bisulfite sequencing PCR results showed that there were no significant change of WT1 gene methylation in cloned pig C1 and C2，and the methylation level of the both CpG inslands in FGF9 gene of cloned pig C1 was much higher than those in N（94.54% vs 18.18%，71.11% vs 26.67%）.The cloned pig C2 was not significant.Results indicated that the abnormal expression of WT1 gene may be one of the reasons for cryptorchidism of the cloned pigs，but the WT1 gene methylation had no relation on this abnormal expression.The change of DNA methylation reprogramming of FGF9 gene occurred in the testis of cloned pigs，which effected normal expression levels of the FGF9 gene，suggested that it may be an important factor for the occurrence of cryptorchidism in the cloned pigs.

CLC Number:

S828.2

LI Fang-fang，DAI Jian-jun，WU Cai-feng,ZHANG Shu-shan，ZHANG De-fu，GU Xiao-long，WU Yun-long，WANG Xin. mRNA Expression and DNA Methylation Status of WT1 and FGF9 Genes in Cryptorchidism of Cloned Pigs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 918-925.

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mRNA Expression and DNA Methylation Status of WT1 and FGF9 Genes in Cryptorchidism of Cloned Pigs

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